eclipse a4f system  (Waters Corporation)

Journal: Cancer Research

Article Title: A Tetravalent Bispecific Antibody Selectively Inhibits Diverse FGFR3 Oncogenic Variants

doi: 10.1158/0008-5472.can-23-3195

Figure Lengend Snippet: Figure 2. The bispecific arms of R3-Altibody form cis complexes with FGFR3-ECD. A, HDX epitope mapping shows that Ab1 (Fab component of R3-Altibody) interacts with Ig-like domain 3, whereas Ab3 (N-term scFv component of R3-Altibody) interacts with Ig-like domain 1. Magenta, peptides that showed differences in deuterium uptake ΔD% ≥ 25%; blue, 25% >ΔD ≥ 20%. Structure of FGFR3 D2–D3 was adopted from PDB 1RY7, model of FGFR3 D1 was adopted from AlphaFold structure prediction (AF-P22607-F1- model_v4). B, R3-Altibody predominantly forms a 1:2 complex (calculated molecular weight 302 kDa) with monomeric FGFR3b-ECD when mixed at 1:2 µmol/L ratio (left). The molar masses of free FGFR3-ECD, R3-Altibody, and R3-Altibody in complex with FGFR3b-ECD were determined by A4F-MALS. The experimentally determined molar masses are indicated by horizon lines. Left, two models of the 1:2 complex are depicted as cis vs. trans binding. Right, the molar masses of the free FGFR3-ECD, scFv-Fab arm from R3-Altibody, and the scFv-Fab arm in complex with FGFR3b-ECD were determined by SEC-MALS. The chromatograms show traces representing scFv-Fab:hFGFR3b- ECD mixed at ratios of 1:3 (green), 1:1 (red), and 3:1 (blue). Molecular mass and pictogram of free scFv-Fab, FGFR3b-ECD, and the complex of scFv-Fab bound to FGFR3-ECD are shown above the corresponding peaks. The minor species of ∼247 kDa is consistent with a 1:2 complex (in cis mode) between residual unreduced 2 + 2 (scFv-Fabʹ)2 in our preparation and FGFR3b-ECD. (A, Created with PyMOL.)

Article Snippet: All samples were incubated at ambient temperature for 2170 Cancer Res; 84(13) July 1, 2024 CANCER RESEARCH ow nloaded from http://aacrjournals.org/cancerres/article-pdf/84/13/2169/3469387/can-23-3195.pdf by guest on 13 Septem ber 2024 2 hours and injected into an Eclipse DualTec A4F separation system coupled to an Agilent 1200 HPLC with DAWN laser light scattering instrument and an Optilab T-rEX differential refractometer detector (Wyatt Technology).

Techniques: Molecular Weight, Binding Assay

Journal: Polymer Chemistry

Article Title: Stereoregular polymerization of phenylacetylene by alkynyl and methyl rhodium(I) complexes with functionalized phosphine ligands: linear vs branched poly(phenylacetylene)s

doi: 10.1039/d4py00497c

Figure Lengend Snippet: Fig. 3 SEC chromatograms: light scattering detector response (90 degrees) (blue) and differential refractometer response (red), and MM (molar mass) vs. elution volume plot for a PPA sample prepared with the catalyst [Rh(CuC-Ph)(nbd){Ph2P(CH2)3NMe2}2] (1) in THF analyzed by: (a) SEC-MALS and (b) A4F-MALS. Log–log plot of the radius of gyration (rg) vs. MM (blue) and linear fit (red) of the same sample analyzed by: (c) SEC-MALS and (d) A4F-MALS.

Article Snippet: The instrumental setup consisted of a Wyatt Technology Corporation A4F system Eclipse 3+, a MALS photometer EOS (Wyatt Technology Corporation) and a refractive index (RI) detector 410 (Waters).

Techniques:

Journal: Polymer Chemistry

Article Title: Stereoregular polymerization of phenylacetylene by alkynyl and methyl rhodium(I) complexes with functionalized phosphine ligands: linear vs branched poly(phenylacetylene)s

doi: 10.1039/d4py00497c

Figure Lengend Snippet: Fig. 4 SEC chromatograms: light scattering detector response (90 degrees) (blue) and differential refractometer response (red), and MM (molar mass) vs. elution volume plot for a PPA sample prepared with the catalyst [Rh(CuC-Ph)(cod){Ph2P(CH2)3OEt}] (4) in THF analyzed by: (a) SEC-MALS and (b) A4F-MALS. Log–log plot of the radius of gyration (rg) vs. MM (blue) and linear fit (red) of the same sample analyzed by: (c) SEC-MALS and (d) A4F-MALS.

Article Snippet: The instrumental setup consisted of a Wyatt Technology Corporation A4F system Eclipse 3+, a MALS photometer EOS (Wyatt Technology Corporation) and a refractive index (RI) detector 410 (Waters).

Techniques:

Journal: Polymer Chemistry

Article Title: Stereoregular polymerization of phenylacetylene by alkynyl and methyl rhodium(I) complexes with functionalized phosphine ligands: linear vs branched poly(phenylacetylene)s

doi: 10.1039/d4py00497c

Figure Lengend Snippet: Fig. 7 Cumulative molar mass distribution (black line) and branch units per macromolecule (B) (red dots) vs. MM determined by A4F-MALS for a PPA sample prepared with the catalyst [Rh(CuC-Ph)(nbd){Ph2P (CH2)3NMe2}2] (1).

Article Snippet: The instrumental setup consisted of a Wyatt Technology Corporation A4F system Eclipse 3+, a MALS photometer EOS (Wyatt Technology Corporation) and a refractive index (RI) detector 410 (Waters).

Techniques:

Journal: PLoS ONE

Article Title: Scalable production and complete biophysical characterization of poly(ethylene glycol) surface conjugated liposome encapsulated hemoglobin (PEG-LEH)

doi: 10.1371/journal.pone.0269939

Figure Lengend Snippet: A typical PEG-LEH nanoparticle absolute size distribution plot generated using multi-angle static light scattering data obtained from a Dawn Heleos ® MASLS photometer. Nanoparticle fractionation was achieved using an Eclipse ® A4F system.

Article Snippet: An Eclipse ® asymmetric flow field-flow fractionator (A4F) (Wyatt Technology Corp., Santa Barbara, CA) connected in series to an 18-angle Dawn Heleos ® multi-angle static light scattering (MASLS) photometer (Wyatt Technology Corp., Santa Barbara, CA) was used to measure the absolute size distribution of PEG-LEH nanoparticles as described in the literature [ , ].

Techniques: Generated, Fractionation